Recombinant Human Cannabinoid Receptor I Protein (Fc Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
CB1 (Met1-Gln116) + hFc (Glu99-Ala330)
Tag
C-terminal Human Fc Tag
Molecular Weight
The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide.The apparent molecular mass of CB1-hFc is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze / thaw cycles.
Figure 2: Recombinant Human Cannabinoid Receptor I Protein (Fc Tag) (A318228)
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human Cannabinoid Receptor I Protein (Fc Tag) (A318228) which can bind Anti-Cannabinoid Receptor I Antibody [DM144] - Azide free (A318564) in a linear range of 3.20-16 µg/ml.
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