Recombinant Human CD80 Protein (Fc Tag) (A318255)

Name: Recombinant Human CD80 Protein (Fc Tag)
Brand: Antibodies.com
SKU: A318255
Price: 565.0 USD
Availability: InStock

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$565

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Specifications

Name

Recombinant Human CD80 Protein (Fc Tag)

Applications

ELISA, SDS-PAGE

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment.

Sequence

B7-1 (Val35-Asn242) + hFc (Glu99-Ala330)

Tag

C-terminal Human Fc Tag

Molecular Weight

The protein has a predicted molecular mass of 50.0 kDa after removal of the signal peptide.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, B7-1 antigen, B7.1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CD80 molecule, CD80_HUMAN, Costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: SDS-PAGE - Recombinant Human CD80 Protein (Fc Tag) (A318255)

SDS-PAGE of Recombinant Human CD80 Protein (Fc Tag) (A318255) under reducing conditions.

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Figure 2: Recombinant Human CD80 Protein (Fc Tag) (A318255)

ELISA plates were pre-coated with Recombinant Human CD80 Protein (Fc Tag) (A318255) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) in a linear range of 0.048-2.094 ng/ml.

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Figure 3: Recombinant Human CD80 Protein (Fc Tag) (A318255)

ELISA plates were pre-coated with Recombinant Human CD80 Protein (Fc Tag) (A318255) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 125-4000 ng/ml.

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Figure 4: Flow Cytometry - Recombinant Human CD80 Protein (Fc Tag) (A318255)

HEK293 cell line was transfected with: Irrelevant protein (B) and human CD28 (A). Cells were surface stained with 1µg/ml of Recombinant Human CD80 Protein (Fc Tag) (A318255) followed by Anti-Human IgG Antibody (Alexa 488).