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Recombinant Human IL-5 Protein (Fc Tag) (A318201)

SDS-PAGE - Recombinant Human IL-5 Protein (Fc Tag) (A318201) - Antibodies.com Flow Cytometry - Recombinant Human IL-5 Protein (Fc Tag) (A318201) - Antibodies.com ELISA - Recombinant Human IL-5 Protein (Fc Tag) (A318201) - Antibodies.com
$515
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$40 to United States
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7-8 Business Days

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Name
Recombinant Human IL-5 Protein (Fc Tag)
Applications
ELISA, SDS-PAGE, Flow Cytometry
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
IL5 (Ile20-Ser134) + hFc (Glu99-Ala330)
Tag
C-terminal Human Fc Tag
Molecular Weight
The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of IL5-hFc is approximately 35-55 kDa due to glycosylation.
Conjugate

Unconjugated

Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor, Colony stimulating factor eosinophil, EDF , Eosinophil differentiation factor, IL 5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin 5 (colony stimulating factor, eosinophil), Interleukin-5, Interleukin5, T cell replacing factor, T-cell replacing factor, TRF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human IL-5 Protein (Fc Tag) (A318201) - Antibodies.comEnlarge Image
Figure 1: SDS-PAGE - Recombinant Human IL-5 Protein (Fc Tag) (A318201)
Recombinant Human IL-5 Protein (Fc Tag) (A318201) on SDS-PAGE under reducing conditions.
Flow Cytometry - Recombinant Human IL-5 Protein (Fc Tag) (A318201) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Recombinant Human IL-5 Protein (Fc Tag) (A318201)
Flow cytometry analysis with Recombinant Human IL-5 Protein (Fc Tag) (A318201) at 15 µg/ml on Expi293 cells transfected with Human IL5RA protein (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
ELISA - Recombinant Human IL-5 Protein (Fc Tag) (A318201) - Antibodies.comEnlarge Image
Figure 3: Recombinant Human IL-5 Protein (Fc Tag) (A318201)
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human IL-5 Protein (Fc Tag) (A318201) which can bind Anti-IL-5 Chimeric Antibody [DMC274] - Azide free (A318784) in a linear range of 0.64-16 µg/ml.
Publishing research using Recombinant Human IL-5 Protein (Fc Tag) (A318201)? Please let us know so that we can list the citation on this page.

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