Recombinant Human TNFRSF14/HVEM Protein (6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
HVEM (Leu39-Val202) + 6xHis Tag
Tag
C-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide. The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 2: Recombinant Human TNFRSF14/HVEM Protein (6xHis Tag) (A318284)
ELISA plates were pre-coated with Recombinant Human TNFRSF14/HVEM Protein (6×His Tag) (A318284) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD272/BTLA Protein (Fc Chimera 6xHis Tag) (A318362) in a linear range of 0.64-80 ng/ml.
Figure 3: Recombinant Human TNFRSF14/HVEM Protein (6xHis Tag) (A318284)
ELISA plates were pre-coated with Recombinant Human TNFRSF14/HVEM Protein (6×His Tag) (A318284) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD160 Protein (Fc Tag) (A318357) in a linear range of 1.28-160 ng/ml.
Figure 4: Recombinant Human TNFRSF14/HVEM Protein (6xHis Tag) (A318284)
ELISA plates were pre-coated with Recombinant Human TNFRSF14/HVEM Protein (6×His Tag) (A318284) at 2 µg/ml (100 µl/well) which can bind Recombinant Human LIGHT/TNFSF14 Protein (Fc Tag) (A318361) in a linear range of 0.32-40 ng/ml.
Figure 5: Flow Cytometry - Recombinant Human TNFRSF14/HVEM Protein (6xHis Tag) (A318284)
Flow cytometry analysis of 15µg/ml of Recombinant Human TNFRSF14/HVEM Protein (6×His Tag) (A318284) on Expi293 cells transfected with human BTLA (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 6: Flow Cytometry - Recombinant Human TNFRSF14/HVEM Protein (6xHis Tag) (A318284)
Flow cytometry analysis of 15µg/ml of Recombinant Human TNFRSF14/HVEM Protein (6×His Tag) (A318284) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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