Anti-RING1 Antibody [ARC2278] (A305619)

Name: Anti-RING1 Antibody [ARC2278]
Brand: Antibodies.com
SKU: A305619
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2278] antibody to RING1 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-RING1 Antibody [ARC2278]

Description

Rabbit monoclonal [ARC2278] antibody to RING1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RING1A A (Q06587).

Sequence

DPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2278

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 ubiquitin-protein ligase RING1, Polycomb complex protein RING1, Really interesting new gene 1 protein, RING finger protein 1, RING1A, RING1_HUMAN, Rnf1, Transcription repressor Ring1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-RING1 Antibody [ARC2278] (A305619)

Western blot analysis of extracts of various cell lines, using Anti-RING1 Antibody [ARC2278] (A305619) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-RING1 Antibody [ARC2278] (A305619)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-RING1 Antibody [ARC2278] (A305619) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-RING1 Antibody [ARC2278] (A305619)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-RING1 Antibody [ARC2278] (A305619). This Western blot was performed on the immunoprecipitate using Anti-RING1 Antibody [ARC2278] (A305619) at a dilution of 1:1000.

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