Anti-RIP Antibody (A10146)

Name: Anti-RIP Antibody
Brand: Antibodies.com
SKU: A10146
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to RIP for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-RIP Antibody

Description

Rabbit polyclonal antibody to RIP.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human RIPK1/RIP (NP_003795.2).

Sequence

GMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

75 kDa / 38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cell death protein RIP, FLJ39204, OTTHUMP00000039163, Receptor (TNFRSF) interacting serine threonine kinase 1, Receptor interacting protein, receptor interacting protein 1, Receptor interacting protein kinase 1, Receptor interacting serine threonine protein kinase 1, Receptor TNFRSF interacting serine threonine kinase 1, Receptor-interacting protein 1, Receptor-interacting serine/threonine-protein kinase 1, Rinp, RIP 1, Rip-1, RIP1, RIPK 1, Ripk1, RIPK1_HUMAN, Serine threonine protein kinase RIP, Serine/threonine-protein kinase RIP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-RIP Antibody (A10146)

Western blot analysis of extracts of C6 cells, using Anti-RIP Antibody (A10146) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Western Blot - Anti-RIP Antibody (A10146)

Western blot analysis of extracts of various cell lines, using Anti-RIP Antibody (A10146) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-RIP Antibody (A10146)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-RIP Antibody (A10146) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-RIP Antibody (A10146)

Immunofluorescence analysis of NIH/3T3 cells using Anti-RIP Antibody (A10146) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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