Anti-RNF7 Antibody [ARC3065] (A309459)

Name: Anti-RNF7 Antibody [ARC3065]
Brand: Antibodies.com
SKU: A309459
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC3065] antibody to RNF7 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-RNF7 Antibody [ARC3065]

Description

Rabbit monoclonal [ARC3065] antibody to RNF7.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of Human RNF7.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC3065

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

13 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CKBBP 1, CKBBP1, CKII beta binding protein 1, CKII beta-binding protein 1, Rbx 2, Rbx2, RBX2_HUMAN, Regulator of cullins 2, RING box protein 2, RING finger protein 7, RING-box protein 2, RNF 7, ROC 2, ROC2, SAG, Sensitive to apoptosis gene, Sensitive to apoptosis gene protein, Zinc RING finger protein SAG

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-RNF7 Antibody [ARC3065] (A309459)

Western blot analysis of extracts of various cell lines, using Anti-RNF7 Antibody [ARC3065] (A309459) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-RNF7 Antibody [ARC3065] (A309459)

Western blot analysis of extracts of DU145 cells, using Anti-RNF7 Antibody [ARC3065] (A309459) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-RNF7 Antibody [ARC3065] (A309459)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-RNF7 Antibody [ARC3065] (A309459) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-RNF7 Antibody [ARC3065] (A309459)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-RNF7 Antibody [ARC3065] (A309459) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.