Anti-RNF8 Antibody [ARC58373] (A307349)

Name: Anti-RNF8 Antibody [ARC58373]
Brand: Antibodies.com
SKU: A307349

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This product is discontinued

Anti-RNF8 Antibody [ARC58373] (A307349) has been discontinued and is no longer available.

View all Anti-RNF8 Antibodies.

Specifications

Name

Anti-RNF8 Antibody [ARC58373]

Description

Rabbit monoclonal [ARC58373] antibody to RNF8.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF8 (NP_003949.1).

Sequence

MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQLVSKICPLMISRNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQGDYIQLGVPLENKENAEYEYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVSCESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSSASQRSLQMFKVTMSRILRLK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58373

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C3HC4 type zinc finger protein, E3 ubiquitin protein ligase RNF8, E3 ubiquitin-protein ligase RNF8, FLJ12013, KIAA0646, Ring finger protein (C3HC4 type) 8, RING finger protein 8, Ring finger protein 8, E3 ubiquitin protein ligase, RNF 8, RNF8_HUMAN, UBC13/UEV interacting ring finger protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-RNF8 Antibody [ARC58373] (A307349)

Western blot analysis of various lysates, using Anti-RNF8 Antibody [ARC58373] (A307349) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-RNF8 Antibody [ARC58373] (A307349)

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Figure 3: Immunohistochemistry - Anti-RNF8 Antibody [ARC58373] (A307349)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-RNF8 Antibody [ARC58373] (A307349) at dilution of1:300(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-RNF8 Antibody [ARC58373] (A307349)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-RNF8 Antibody [ARC58373] (A307349) at dilution of1:300(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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