Anti-ROCK1 Antibody (A12783)

Name: Anti-ROCK1 Antibody
Brand: Antibodies.com
SKU: A12783
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to ROCK1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-ROCK1 Antibody

Description

Rabbit polyclonal antibody to ROCK1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Sequence

YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

158 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

coiled-coil-containing protein kinase 1, coiled-coil-containing protein kinase I, MGC131603, MGC43611, p160 Rhoassociated coiled coil-forming protein kinase, p160 ROCK-1, p160 ROCK1, p160ROCK, PRO0435, Renal carcinoma antigen NY REN 35, Renal carcinoma antigen NY-REN-35, Rho associated coiled coil containing protein kinase 1, Rho associated protein kinase 1, Rho kinase, Rho-alpha kinase, Rho-associated, Rho-associated protein kinase 1, ROCK I, ROCK-I, ROCK1_HUMAN, Rok, rokalpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of various lysates, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of C6, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of extracts from wild type(WT) and ROCK1 knockout (KO) 293T cells, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-ROCK1 Antibody (A12783)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-ROCK1 Antibody (A12783) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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