Anti-ROCK2 Antibody [ARC0744] (A308106)

Name: Anti-ROCK2 Antibody [ARC0744]
Brand: Antibodies.com
SKU: A308106
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0744] antibody to ROCK2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ROCK2 Antibody [ARC0744]

Description

Rabbit monoclonal [ARC0744] antibody to ROCK2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1289-1388 of human ROCK2 (O75116).

Sequence

PALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0744

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

coiled-coil-containing protein kinase 2, KIAA0619, p164 ROCK 2, p164 ROCK-2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho associated, coiled coil containing protein kinase II, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, ROCK 2, Rock II, Rock2m, ROCK2_HUMAN, ROK alpha, ROKalpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-ROCK2 Antibody [ARC0744] (A308106)

Western blot analysis of extracts of various cell lines, using Anti-ROCK2 Antibody [ARC0744] (A308106) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-ROCK2 Antibody [ARC0744] (A308106)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-ROCK2 Antibody [ARC0744] (A308106) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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