Anti-RPE65 Antibody [ARC1659] (A308904)

Name: Anti-RPE65 Antibody [ARC1659]
Brand: Antibodies.com
SKU: A308904
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC1659] antibody to RPE65 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-RPE65 Antibody [ARC1659]

Description

Rabbit monoclonal [ARC1659] antibody to RPE65.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RPE65 (Q16518).

Sequence

TIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1659

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

All-trans-retinyl-palmitate hydrolase, LCA 2, LCA2, Leber congenital amaurosis, mRPE 65, mRPE65, p63, rd 12, rd12, Retinal pigment epithelium specific 61 kDa protein, Retinal pigment epithelium specific 65 kDa protein, Retinal pigment epithelium specific protein, Retinal pigment epithelium specific protein 65kDa, Retinal pigment epithelium-specific 65 kDa protein, Retinitis pigmentosa 20, Retinoid isomerohydrolase, Retinol isomerase, RP 20, RP20, RPE 65, RPE65_HUMAN, sRPE 65, sRPE65

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-RPE65 Antibody [ARC1659] (A308904)

Western blot analysis of extracts of Rat eye, using Anti-RPE65 Antibody [ARC1659] (A308904) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-RPE65 Antibody [ARC1659] (A308904)

Immunohistochemistry analysis of paraffin-embedded rat retina using Anti-RPE65 Antibody [ARC1659] (A308904) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-RPE65 Antibody [ARC1659] (A308904)

Immunohistochemistry analysis of paraffin-embedded mouse retina using Anti-RPE65 Antibody [ARC1659] (A308904) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunofluorescence - Anti-RPE65 Antibody [ARC1659] (A308904)

Immunofluorescence analysis of rat eye using Anti-RPE65 Antibody [ARC1659] (A308904) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).