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Anti-RPL10A Antibody (A15055)

Western Blot - Anti-RPL10A Antibody (A15055) - Antibodies.com Immunohistochemistry - Anti-RPL10A Antibody (A15055) - Antibodies.com Immunohistochemistry - Anti-RPL10A Antibody (A15055) - Antibodies.com Immunofluorescence - Anti-RPL10A Antibody (A15055) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to RPL10A for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-RPL10A Antibody
Description
Rabbit polyclonal antibody to RPL10A.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human RPL10A (NP_009035.3).
Sequence
MSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
60S ribosomal protein L10a, CSA 19, CSA-19, CSA19, L10A, NEDD 6, NEDD-6, NEDD6, neural precursor cell expressed developmentally down regulated protein 6, Neural precursor cell expressed developmentally down-regulated protein 6, Protein NEDD6, Ribosomal protein L10a, RL10A_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RPL10A Antibody (A15055) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RPL10A Antibody (A15055)
Western blot analysis of extracts of various cell lines, using Anti-RPL10A Antibody (A15055) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-RPL10A Antibody (A15055) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-RPL10A Antibody (A15055)
Immunohistochemistry analysis of paraffin-embedded rat cerebellum using Anti-RPL10A Antibody (A15055) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-RPL10A Antibody (A15055) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-RPL10A Antibody (A15055)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-RPL10A Antibody (A15055) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-RPL10A Antibody (A15055) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-RPL10A Antibody (A15055)
Immunofluorescence analysis of C6 cells using Anti-RPL10A Antibody (A15055) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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