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Anti-Reptin / TIP49B / RUVB2 Antibody (A13734)

Western Blot - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) - Antibodies.com Immunofluorescence - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) - Antibodies.com Western Blot - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) - Antibodies.com
This product is discontinued

Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Reptin / TIP49B / RUVB2 Antibody
Description
Rabbit polyclonal antibody to Reptin / TIP49B / RUVB2.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human Reptin/Reptin/RUVBL2 (NP_006657.1).
Sequence
MATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAFLFNELKGETMDTS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) - Antibodies.comEnlarge Image

Western Blot - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734)

Western blot analysis of extracts of various cell lines, using Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734)

Immunofluorescence analysis of U2OS cells using Anti-Reptin / TIP49B / RUVB2 Antibody (A13734). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) - Antibodies.comEnlarge Image

Western Blot - Anti-Reptin / TIP49B / RUVB2 Antibody (A13734)

Immunoprecipitation analysis of 200µg extracts of K-562 cells using 3µg of Anti-Reptin / TIP49B / RUVB2 Antibody (A13734). This Western blot was performed on the immunoprecipitate using Anti-Reptin / TIP49B / RUVB2 Antibody (A13734) at a dilution of 1:1000.