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Anti-RYK Antibody (A305053)

Western Blot - Anti-RYK Antibody (A305053) - Antibodies.com Immunohistochemistry - Anti-RYK Antibody (A305053) - Antibodies.com
$500
Product Datasheet

Rabbit polyclonal antibody to RYK for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-RYK Antibody
Description
Rabbit polyclonal antibody to RYK.
Specificity
Band at 37 kDa is potential degradation product.
Applications
WB, IHC
Dilutions
WB: 1:1,000, IHC: 1:50
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide of Human RYK (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
67.5 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
D3S3195, ERK 3, Hydroxyaryl protein kinase, JTK 5, JTK 5A, JTK5, JTK5A, JTK5A protein tyrosine kinase, receptor like tyrosine kinase, receptor like tyrosine kinase precursor, RYK 1, RYK1, RYK_HUMAN, Tyrosine protein kinase RYK, Tyrosine protein kinase RYK precursor, Tyrosine-protein kinase RYK, Vik
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RYK Antibody (A305053) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RYK Antibody (A305053)
Western blot analysis of mouse brain lysate showing detection of ~67.5 kDa RYK protein using Anti-RYK Antibody (A305053) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.
Immunohistochemistry - Anti-RYK Antibody (A305053) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-RYK Antibody (A305053)
Immunohistochemistry analysis of human breast cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-RYK Antibody (A305053) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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