Anti-S100 alpha Antibody [ARC59163] (A309671)

Name: Anti-S100 alpha Antibody [ARC59163]
Brand: Antibodies.com
SKU: A309671
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC59163] antibody to S100 alpha for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-S100 alpha Antibody [ARC59163]

Description

Rabbit monoclonal [ARC59163] antibody to S100 alpha.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human S100/S100A1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59163

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

12 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Bpb, NEF, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100, S100 Alpha Chain, S100 Beta Chain, S100 Calcium Binding Protein A1, S100 Calcium Binding Protein B, S100 Calcium Binding Protein Beta Neural, S100 calcium-binding protein A1, S100 protein alpha polypeptide, S100A, s100a1, S10A1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-S100 alpha Antibody [ARC59163] (A309671)

Western blot analysis of various lysates, using Anti-S100 alpha Antibody [ARC59163] (A309671) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-S100 alpha Antibody [ARC59163] (A309671)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-S100 alpha Antibody [ARC59163] (A309671) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-S100 alpha Antibody [ARC59163] (A309671)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-S100 alpha Antibody [ARC59163] (A309671) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-S100 alpha Antibody [ARC59163] (A309671)

Immunofluorescence analysis of HepG2 cells using Anti-S100 alpha Antibody [ARC59163] (A309671) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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