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Anti-S100A4 Antibody (A13562)

Name
Anti-S100A4 Antibody
Description
Rabbit polyclonal antibody to S100A4.
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).
Sequence
MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
12kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
18A2, 42A, calcium Placental protein, Calvasculin, CAPL, Fibroblast specific protein, Fibroblast specific protein 1, Fibroblast specific protein 1 (FSP1), FSP1, Leukemia multidrug resistance associated protein, Malignant transformation suppression 1, Malignant transformation suppression 1 (MTS1), Metastasin, MTS1, OTTHUMP00000015467, OTTHUMP00000015468, P9KA, PEL98, Placental calcium-binding protein, Protein Mts1, Protein S100 A4, Protein S100-A4, S100 calcium binding protein A4, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S10A4_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-S100A4 Antibody (A1631) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-S100A4 Antibody (A13562)
Western blot analysis of extracts of various cell lines, using Anti-S100A4 Antibody (A1631) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Western Blot - Anti-S100A4 Antibody (A1631) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-S100A4 Antibody (A13562)
Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using Anti-S100A4 Antibody (A1631) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-S100A4 Antibody (A1631) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-S100A4 Antibody (A13562)
Immunohistochemistry of paraffin-embedded rat brain using Anti-S100A4 Antibody (A1631) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-S100A4 Antibody (A1631) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-S100A4 Antibody (A13562)
Immunohistochemistry of paraffin-embedded human mammary gland using Anti-S100A4 Antibody (A1631) at dilution of 1:100 (40x lens).
Publishing research using Anti-S100A4 Antibody (A13562)? Please let us know so that we can list the citation on this page.

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