Anti-S100A9 Antibody [ARC55864] (A307900)

Name: Anti-S100A9 Antibody [ARC55864]
Brand: Antibodies.com
SKU: A307900

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This product is discontinued

Anti-S100A9 Antibody [ARC55864] (A307900) has been discontinued and is no longer available.

View all Anti-S100A9 Antibodies.

Specifications

Name

Anti-S100A9 Antibody [ARC55864]

Description

Rabbit monoclonal [ARC55864] antibody to S100A9.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human S100A9 (NP_002956.1).

Sequence

MTCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55864

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

60B8AG, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG, CGLB, Cystic fibrosis antigen B, L1AG, Leukocyte L1 complex heavy chain, LIAG, MAC387, MIF, Migration inhibitory factor related protein 14, Migration inhibitory factor-related protein 14, MRP 14, MRP-14, MRP14, Myeloid-related protein 14, NIF, OTTHUMP00000015331, p14, Protein S100-A9, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S10A9_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-S100A9 Antibody [ARC55864] (A307900)

Western blot analysis of various lysates, using Anti-S100A9 Antibody [ARC55864] (A307900) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-S100A9 Antibody [ARC55864] (A307900)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S100A9 Antibody [ARC55864] (A307900) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-S100A9 Antibody [ARC55864] (A307900)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-S100A9 Antibody [ARC55864] (A307900) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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