Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461)

Name: Anti-S100A9 Antibody [ARC55864 + ARC55869]
Brand: Antibodies.com
SKU: A309461

Enlarge Image

This product is discontinued

Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461) has been discontinued and is no longer available.

View all Anti-S100A9 Antibodies.

Specifications

Name

Anti-S100A9 Antibody [ARC55864 + ARC55869]

Description

Rabbit Oligoclonal [ARC55864 + ARC55869] antibody to S100A9.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human S100A9.

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC55864 + ARC55869

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

60B8AG, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG, CGLB, Cystic fibrosis antigen B, L1AG, Leukocyte L1 complex heavy chain, LIAG, MAC387, MIF, Migration inhibitory factor related protein 14, Migration inhibitory factor-related protein 14, MRP 14, MRP-14, MRP14, Myeloid-related protein 14, NIF, OTTHUMP00000015331, p14, Protein S100-A9, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S10A9_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461)

Western blot analysis of various lysates, using Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.

Enlarge Image

Figure 2: Western Blot - Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461)

Enlarge Image

Figure 3: Immunohistochemistry - Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461)

Immunohistochemistry analysis of paraffin-embedded human cervix cancer tissue using Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461) at a dilution of 1:5000/15000/45000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S100A9 Antibody [ARC55864 + ARC55869] (A309461) at a dilution of 1:5000/15000/45000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.