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Anti-SAM Antibody [84-3] (A51742)

Titration Curve - Anti-SAM Antibody from Arthus Biosystems (MAH00202) - Antibodies.com Standard Curve - Anti-SAM Antibody from Arthus Biosystems (MAH00202) - Antibodies.com
$1,695

Mouse monoclonal (84-3) antibody to S-Adenosylmethionine (HRP) for cELISA.

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$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-SAM Antibody [84-3]
Description
Mouse monoclonal (84-3) antibody to S-Adenosylmethionine (HRP).
Specificity
This antibody shows the following reactivities with related compounds: S-Adenosylmethionine: 100%, S-Adenosylhomocysteine: < 1%, Adenosine: < 1%, L-Methionine: < 1%, Methythioadenosine (MTA): < 1%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%.
Applications
ELISA">cELISA
Dilutions
cELISA: 1:5,000 - 1:50,000 (depending on the amount of coating).
Immunogen
S-Adenosylmethionine analog Aza-SAM conjugated to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
84-3
Isotype
IgG2b
Conjugate

HRP

Purification
Affinity chromatography
Purity
> 95%
Product Form
Liquid
Formulation
Supplied in 10mM PBS, pH 7.4, with 150mM NaCl, 0.1% Proclin, 50% Glycerol, and 10mg/ml BSA.
Storage
Store at 4°C, -20°C long term storage.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Titration Curve - Anti-SAM Antibody from Arthus Biosystems (MAH00202) - Antibodies.comEnlarge Image
Figure 1: Anti-SAM Antibody [84-3] (A51742)
Titer of MAH00202. The 0.25 µl/ml of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. The MAH00202 was serially diluted and added to the wells. After incubation of about 60 minutes, substrates were added and OD450 was measured.
Standard Curve - Anti-SAM Antibody from Arthus Biosystems (MAH00202) - Antibodies.comEnlarge Image
Figure 2: Standard Curve - Anti-SAM Antibody [84-3] (A51742)
The standard curve with MAH00202 in direct competitive ELISA using 4-parameter logistic non-linear regression model. The 0.25 µg/ml of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. Serial dilution of SAM standard (Cat # AST00201) was added along with diluted MAH00202 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.
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