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Anti-SAPK (phospho Tyr185) Antibody (A94395)

ELISA - Anti-SAPK (phospho Tyr185) Antibody (P12-1175) - Antibodies.com Western Blot - Anti-SAPK (phospho Tyr185) Antibody (P12-1175) - Antibodies.com
This product is discontinued

Anti-SAPK (phospho Tyr185) Antibody (A94395) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-SAPK (phospho Tyr185) Antibody
Description
Rabbit polyclonal antibody to SAPK (phospho Tyr185).
Specificity
This antibody detects endogenous levels of MAPK8/9/10 only when phosphorylated at Tyr185.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
48kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

ELISA - Anti-SAPK (phospho Tyr185) Antibody (P12-1175) - Antibodies.comEnlarge Image

Anti-SAPK (phospho Tyr185) Antibody (A94395)

ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-SAPK (phospho Tyr185) Antibody.
Western Blot - Anti-SAPK (phospho Tyr185) Antibody (P12-1175) - Antibodies.comEnlarge Image

Western Blot - Anti-SAPK (phospho Tyr185) Antibody (A94395)

Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-SAPK (phospho Tyr185) Antibody.