Anti-SARA Antibody [ARC2863] (A307254)

Name: Anti-SARA Antibody [ARC2863]
Brand: Antibodies.com
SKU: A307254

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This product is discontinued

Anti-SARA Antibody [ARC2863] (A307254) has been discontinued and is no longer available.

View all Anti-SARA Antibodies.

Specifications

Name

Anti-SARA Antibody [ARC2863]

Description

Rabbit monoclonal [ARC2863] antibody to SARA.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SARA (O95405).

Sequence

MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESAVSNESQPQLKVFSLAHSAPLTTEEEDHCANGQDCNLNPEI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2863

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

180 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CG15667, hSARA, LD33044p, Madh-interacting protein, MADHIP, Mothers against decapentaplegic homolog-interacting protein, Novel serine protease, NSP, Receptor activation anchor, SAR1, SARA1, Smad anchor for receptor activation, SMADIP, ZFYV9_HUMAN, ZFYVE9, Zinc finger FYVE domain-containing protein 9

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SARA Antibody [ARC2863] (A307254)

Western blot analysis of extracts of various cell lines, using Anti-SARA Antibody [ARC2863] (A307254) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-SARA Antibody [ARC2863] (A307254)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SARA Antibody [ARC2863] (A307254) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SARA Antibody [ARC2863] (A307254)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-SARA Antibody [ARC2863] (A307254) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SARA Antibody [ARC2863] (A307254)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SARA Antibody [ARC2863] (A307254) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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