Anti-SATB2 Antibody [ARC50977] (A306174)

Name: Anti-SATB2 Antibody [ARC50977]
Brand: Antibodies.com
SKU: A306174

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This product is discontinued

Anti-SATB2 Antibody [ARC50977] (A306174) has been discontinued and is no longer available.

View all Anti-SATB2 Antibodies.

Specifications

Name

Anti-SATB2 Antibody [ARC50977]

Description

Rabbit monoclonal [ARC50977] antibody to SATB2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:1,000-1:5,000, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 634-733 of human SATB2 (NP_001165980.1).

Sequence

HDVGLYPDQEAIHTLSAQLDLPKHTIIKFFQNQRYHVKHHGKLKEHLGSAVDVAEYKDEELLTESEENDSEEGSEEMYKVEAEEENADKSKAAPAEIDQR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50977

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA binding protein SATB2, DNA-binding protein SATB2, FLJ21474, FLJ32076, GLSS, KIAA1034, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, SATB2_HUMAN, Special AT rich sequence binding protein 2, Special AT-rich sequence-binding protein 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SATB2 Antibody [ARC50977] (A306174)

Western blot analysis of extracts of various cell lines, using Anti-SATB2 Antibody [ARC50977] (A306174) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-SATB2 Antibody [ARC50977] (A306174)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-SATB2 Antibody [ARC50977] (A306174) at a dilution of 1:2000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SATB2 Antibody [ARC50977] (A306174)

Immunohistochemistry analysis of paraffin-embedded human pancreas (negative control sample) using Anti-SATB2 Antibody [ARC50977] (A306174) at a dilution of 1:2000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SATB2 Antibody [ARC50977] (A306174)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SATB2 Antibody [ARC50977] (A306174) at a dilution of 1:2000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.