Anti-SCAF4 Antibody (A308954)

Name: Anti-SCAF4 Antibody
Brand: Antibodies.com
SKU: A308954
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SCAF4 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SCAF4 Antibody

Description

Rabbit polyclonal antibody to SCAF4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 170-248 of human SCAF4 (NP_065757.1).

Sequence

TPNSVPAVPQLPSSDAFAAVAQLFQTTQGQQLQQILQTFQQPPKPQSPALDNAVMAQVQAITAQLKTTPTQPSEQKAAF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

126 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

arginine/serine-rich 15, CTD-binding SR-like protein RA4, DKFZp434E098, FLJ23364, KIAA1172, Pre mRNA splicing SR protein rA4, SFR15_HUMAN, SFRS15, Splicing factor, Splicing factor arginine/serine rich 15, Splicing factor serine alanine 15, SR like CTD associated factor 4, SR-related and CTD-associated factor 4, SRA4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-SCAF4 Antibody (A308954)

Western blot analysis of extracts of HeLa cells, using Anti-SCAF4 Antibody (A308954) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Western Blot - Anti-SCAF4 Antibody (A308954)

Western blot analysis of extracts of Rat thymus, using Anti-SCAF4 Antibody (A308954) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-SCAF4 Antibody (A308954)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-SCAF4 Antibody (A308954) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SCAF4 Antibody (A308954)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-SCAF4 Antibody (A308954) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.