Anti-Scramblase 1 Antibody [ARC2028] (A307139)

Name: Anti-Scramblase 1 Antibody [ARC2028]
Brand: Antibodies.com
SKU: A307139
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2028] antibody to Scramblase 1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Scramblase 1 Antibody [ARC2028]

Description

Rabbit monoclonal [ARC2028] antibody to Scramblase 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Phospholipid Scramblase 1 (PLSCR1) (O15162).

Sequence

MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2028

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ca(2+) dependent phospholipid scramblase 1, Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MM1 cell-derived transplantability-associated gene 1b, mouse, homolog of, MmTRA1a, MmTRA1b, Nor1, Phospholipid scramblase 1, PL scramblase 1, PLS1_HUMAN, PLSCR 1, Plscr1, Scramblase1, Tra1, Tra1a, Tra1b, Transplantability-associated protein 1, Tras1, Tras2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Scramblase 1 Antibody [ARC2028] (A307139)

Western blot analysis of extracts of HT-29 cells, using Anti-Scramblase 1 Antibody [ARC2028] (A307139) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-Scramblase 1 Antibody [ARC2028] (A307139)

Western blot analysis of extracts of Mouse lung, using Anti-Scramblase 1 Antibody [ARC2028] (A307139) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-Scramblase 1 Antibody [ARC2028] (A307139)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Scramblase 1 Antibody [ARC2028] (A307139) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Scramblase 1 Antibody [ARC2028] (A307139)

Immunofluorescence analysis of C6 cells using Anti-Scramblase 1 Antibody [ARC2028] (A307139) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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