Anti-SDHA Antibody (A14093)

Name: Anti-SDHA Antibody
Brand: Antibodies.com
SKU: A14093
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SDHA for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-SDHA Antibody

Description

Rabbit polyclonal antibody to SDHA.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).

Sequence

ETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CMD1GG, DHSA_HUMAN, Flavoprotein subunit of complex II, Fp, PGL5, SDH 2, SDH1, SDH2, SDHF, Succinate dehydrogenase complex flavoprotein subunit, Succinate dehydrogenase complex flavoprotein subunit A, Succinate dehydrogenase complex flavoprotein subunit precursor, Succinate dehydrogenase complex subunit A, Succinate dehydrogenase complex subunit A flavoprotein, Succinate dehydrogenase complex subunit A flavoprotein (Fp), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SDHA Antibody (A14093)

Western blot analysis of extracts of various cell lines, using Anti-SDHA Antibody (A14093) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-SDHA Antibody (A14093)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SDHA Antibody (A14093) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-SDHA Antibody (A14093)

Immunofluorescence analysis of NIH/3T3 cells using Anti-SDHA Antibody (A14093) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-SDHA Antibody (A14093)

Immunofluorescence analysis of U2OS cells using Anti-SDHA Antibody (A14093) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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