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Anti-Separase Antibody (A88806)

Western Blot - Anti-Separase Antibody (A88806) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Separase for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
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orders@antibodies.com

Name
Anti-Separase Antibody
Description
Rabbit polyclonal antibody to Separase.
Applications
WB, ICC/IF
Dilutions
WB: 1:200-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1220-1500 of human ESPL1 (NP_036423.4).
Sequence
DEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKM
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
233 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Caspase like protein ESPL1, Caspase-like protein ESPL1, ESP 1, ESP1, ESPL 1, Espl1, ESPL1_HUMAN, Extra spindle poles like 1, Extra spindle poles like 1 protein, Extra spindle poles-like 1 protein, FLJ46492, KIAA0165, Separin, Similar to fission yeast cut1and gene, SSE
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Separase Antibody (A88806) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Separase Antibody (A88806)
Western blot analysis of extracts of various cell lines, using Anti-Separase Antibody (A88806) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Publishing research using Anti-Separase Antibody (A88806)? Please let us know so that we can list the citation on this page.

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