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Anti-SEPT5 Antibody (A89872)

Western Blot - Anti-SEPT5 Antibody (A89872) - Antibodies.com Immunohistochemistry - Anti-SEPT5 Antibody (A89872) - Antibodies.com Immunohistochemistry - Anti-SEPT5 Antibody (A89872) - Antibodies.com Immunofluorescence - Anti-SEPT5 Antibody (A89872) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to 38596 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-SEPT5 Antibody
Description
Rabbit polyclonal antibody to 38596.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:1,000-1:5,000, IHC: 1:100-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human Septin 5 (NP_002679.2).
Sequence
MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQQMQDQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
43 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
5 Sep, CDCREL, CDCrel 1, CDCrel-1, CDCREL1, Cell division control related protein 1, Cell division control-related protein 1, H5, HCDCREL 1, OTTHUMP00000197271, OTTHUMP00000197272, OTTHUMP00000197273, OTTHUMP00000197274, OTTHUMP00000197275, OTTHUMP00000197276, OTTHUMP00000197396, OTTHUMP00000197410, Peanut like 1, Peanut like 1 homolog, Peanut like protein 1, Peanut-like protein 1, Platelet glycoprotein Ib beta chain, PNUTL1, SEPT5_HUMAN, Septin 5, Septin-5
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SEPT5 Antibody (A89872) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SEPT5 Antibody (A89872)
Western blot analysis of various lysates, using Anti-SEPT5 Antibody (A89872) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-SEPT5 Antibody (A89872) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-SEPT5 Antibody (A89872)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-SEPT5 Antibody (A89872) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-SEPT5 Antibody (A89872) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-SEPT5 Antibody (A89872)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-SEPT5 Antibody (A89872) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-SEPT5 Antibody (A89872) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-SEPT5 Antibody (A89872)
Immunofluorescence analysis of U-2 OS cells using Anti-SEPT5 Antibody (A89872) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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