Anti-Hsp47 Antibody (A14026)

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This product is discontinued

Anti-Hsp47 Antibody (A14026) has been discontinued and is no longer available.

View all Hsp47 Antibodies.

Specifications

Name

Anti-Hsp47 Antibody

Description

Rabbit polyclonal antibody to Hsp47.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-418 of human HSP47/HSP47/SERPINH1 (NP_001193943.1).

Sequence

SVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3, CBP1, CBP2, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Colligin, Rheumatoid arthritis-related antigen RA-A47, Serpin H1, SERPINH1, SERPINH2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Hsp47 Antibody (A14026)

Western blot analysis of extracts of various cell lines, using Anti-Hsp47 Antibody (A14026) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-Hsp47 Antibody (A14026)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Hsp47 Antibody (A14026) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Hsp47 Antibody (A14026)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Hsp47 Antibody (A14026) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Hsp47 Antibody (A14026)

Immunofluorescence analysis of C6 cells using Anti-Hsp47 Antibody (A14026) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).