Anti-SF3B1 Antibody [ARC1724] (A306918)

Name: Anti-SF3B1 Antibody [ARC1724]
Brand: Antibodies.com
SKU: A306918
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1724] antibody to SF3B1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SF3B1 Antibody [ARC1724]

Description

Rabbit monoclonal [ARC1724] antibody to SF3B1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SF3B1 (O75533).

Sequence

MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1724

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

146 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Hsh 155, MDS, Pre mRNA processing 10, Pre mRNA splicing factor SF3b 155 kDa subunit, PRP 10, PRP10, PRPF 10, PRPF10, SAP 155, SAP155, SF3B 1, SF3b155, Spliceosome associated protein 155, Splicing factor 3B subunit 1, Splicing factor 3b subunit 1 155kDa

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SF3B1 Antibody [ARC1724] (A306918)

Western blot analysis of extracts of various cell lines, using Anti-SF3B1 Antibody [ARC1724] (A306918) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-SF3B1 Antibody [ARC1724] (A306918)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-SF3B1 Antibody [ARC1724] (A306918) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SF3B1 Antibody [ARC1724] (A306918)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-SF3B1 Antibody [ARC1724] (A306918) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SF3B1 Antibody [ARC1724] (A306918)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-SF3B1 Antibody [ARC1724] (A306918) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.