Anti-SHANK3 Antibody [S69] (A304813)

Name: Anti-SHANK3 Antibody [S69]
Brand: Antibodies.com
SKU: A304813
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S69] antibody to SHANK3 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SHANK3 Antibody [S69]

Description

Mouse monoclonal [S69] antibody to SHANK3.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with SHANK1 or SHANK2.

Immunogen

Synthetic peptide amino acids 840-857 of rat Shank3.

Host

Mouse

Clonality

Monoclonal

Clone ID

S69

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~190 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI841104, DEL22q13.3, KIAA1650, Proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, SH3 and multiple ankyrin repeat domains 3, SH3 and multiple ankyrin repeat domains protein 3, SH3/ankyrin domain gene 3, SHAN3_HUMAN, Shank postsynaptic density protein, Shank3b, SPANK 2, SPANK2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SHANK3 Antibody [S69] (A304813)

Western blot analysis of rat brain membrane lysate showing detection of SHANK3 protein using Anti-SHANK3 Antibody [S69] (A304813) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunofluorescence - Anti-SHANK3 Antibody [S69] (A304813)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-SHANK3 Antibody [S69] (A304813), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Borderline positive.

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Figure 3: Immunohistochemistry - Anti-SHANK3 Antibody [S69] (A304813)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-SHANK3 Antibody [S69] (A304813) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Early stages of filaggrin-like and dermal staining.

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Figure 4: Immunofluorescence - Anti-SHANK3 Antibody [S69] (A304813)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-SHANK3 Antibody [S69] (A304813), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 Antibody (D) Composite.

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