Recommended Dilutions
In immunoelectrophoresis: 2 µl 1:8, 1:16 and 1:32 dilutions of pathological serum against 60 µl antiserum. In double radial immunodiffusion: a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.