Anti-SHP1 Antibody [ARC0295] (A308671)

Name: Anti-SHP1 Antibody [ARC0295]
Brand: Antibodies.com
SKU: A308671

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This product is discontinued

Anti-SHP1 Antibody [ARC0295] (A308671) has been discontinued and is no longer available.

View all Anti-SHP1 Antibodies.

Specifications

Name

Anti-SHP1 Antibody [ARC0295]

Description

Rabbit monoclonal [ARC0295] antibody to SHP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 496-595 of human SHP1 (P29350).

Sequence

SGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0295

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

70Z-SHP, EC 3.1.3.48, HCP, HCPH, Hematopoietic cell phosphatase, Hematopoietic cell protein tyrosine phosphatase, Hematopoietic cell protein-tyrosine phosphatase, HPTP1C, Protein tyrosine phosphatase 1C, Protein tyrosine phosphatase non receptor type 6, Protein tyrosine phosphatase SHP1, Protein-tyrosine phosphatase 1C, protein-tyrosine phosphatase SHP 1, Protein-tyrosine phosphatase SHP-1, PTN6_HUMAN, PTP 1C, PTP-1C, PTP1C, Ptpn6, SH PTP 1, SH PTP1, SH-PTP1, SHP 1, SHP 1L, SHP1L, tyrosine protein phosphatase non receptor type 6, Tyrosine-protein phosphatase non-receptor type 6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-SHP1 Antibody [ARC0295] (A308671)

Western blot analysis of extracts of Raji cells, using Anti-SHP1 Antibody [ARC0295] (A308671) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-SHP1 Antibody [ARC0295] (A308671)

Western blot analysis of extracts of various cell lines, using Anti-SHP1 Antibody [ARC0295] (A308671) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-SHP1 Antibody [ARC0295] (A308671)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-SHP1 Antibody [ARC0295] (A308671) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SHP1 Antibody [ARC0295] (A308671)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-SHP1 Antibody [ARC0295] (A308671) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.