Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Name: Anti-SHP2 (phospho Tyr542) Antibody
Brand: Antibodies.com
SKU: A16556
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to SHP2 (phospho Tyr542) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SHP2 (phospho Tyr542) Antibody

Description

Rabbit polyclonal antibody to SHP2 (phospho Tyr542).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y542 of human PTPN11 (NP_002825.3).

Sequence

HEYTN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BPTP3, CFC, JMML, METCDS, MGC14433, NS1, OTTHUMP00000166107, OTTHUMP00000166108, Protein tyrosine phosphatase 2, Protein tyrosine phosphatase 2C, Protein tyrosine phosphatase non receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, PTN11_HUMAN, PTP-1D, PTP-2C, PTP1D, PTP2C, PTPN11, SAP2, SH-PTP2, SH-PTP3, SH2 domain containing protein tyrosine phosphatase 2, SHP 2, SHP-2, SHPTP2, SHPTP3, Syp, Tyrosine-protein phosphatase non-receptor type 11

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Western blot analysis of NIH/3T3, using Anti-SHP2 (phospho Tyr542) Antibody (A16556) at 1:700 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Western blot analysis of C6, using Anti-SHP2 (phospho Tyr542) Antibody (A16556) at 1:700 dilution. C6cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-SHP2 (phospho Tyr542) Antibody (A16556). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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