Anti-SIAH1 Antibody [ARC59737] (A309470)

Name: Anti-SIAH1 Antibody [ARC59737]
Brand: Antibodies.com
SKU: A309470

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This product is discontinued

Anti-SIAH1 Antibody [ARC59737] (A309470) has been discontinued and is no longer available.

View all Anti-SIAH1 Antibodies.

Specifications

Name

Anti-SIAH1 Antibody [ARC59737]

Description

Rabbit monoclonal [ARC59737] antibody to SIAH1.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human SIAH1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59737

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

31 kDa / 35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 ubiquitin-protein ligase SIAH1, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, Seven in absentia homolog 1 (Drosophila), Siah 1, Siah 1a, Siah E3 ubiquitin protein ligase 1, Siah-1, Siah-1a, SIAH1A, SIAH1_HUMAN, Ubiquitin ligase SIAH1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SIAH1 Antibody [ARC59737] (A309470)

Western blot analysis of various lysates, using Anti-SIAH1 Antibody [ARC59737] (A309470) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-SIAH1 Antibody [ARC59737] (A309470)

Western blot analysis of Mouse liver, using Anti-SIAH1 Antibody [ARC59737] (A309470) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-SIAH1 Antibody [ARC59737] (A309470)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-SIAH1 Antibody [ARC59737] (A309470) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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