This antibody recognizes an extracellular epitope of CD170, a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. This antibody cross-reacts with SIGLEC14, whose first two Ig domains are almost identical to those of CD170.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Non-Human Primates

Immunogen

Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1.

Host

Mouse

Clonality

Monoclonal

Clone ID

1A5

Isotype

IgG1

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

CD170, CD170 antigen, CD33 antigen-like 2, CD33L2, mSiglec-F, OB-binding protein 2, OB-BP2, OBBP2, Obesity-binding protein 2, Sialic acid binding Ig-like lectin 5, Sialic acid binding immunoglobulin-like lectin 5, Sialic acid-binding Ig-like lectin 5, Sialic acid-binding Ig-like lectin F, SIGL5_HUMAN, Siglec-5, Siglec-F variant, Siglecf

Isotype Controls