Anti-SIGLEC8 Antibody [ARC52425] (A307227)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Siglec-8 protein, using Anti-SIGLEC8 Antibody [ARC52425] (A307227) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Flow cytometry analysis of 293F cells transfected with Siglec8, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-SIGLEC8 Antibody [ARC52425] (A307227), (10 µg/ml green line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained 293F cells transfected with Siglec8 cells, used as blank control (red line).

Flow cytometry analysis of 293F cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-SIGLEC8 Antibody [ARC52425] (A307227), (10 µg/ml green line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution). Non-fluorescently stained 293F cells, used as blank control (red line).