Anti-SKP2 Antibody [ARC0886] (A307422)

Name: Anti-SKP2 Antibody [ARC0886]
Brand: Antibodies.com
SKU: A307422
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC0886] antibody to SKP2 for WB and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-SKP2 Antibody [ARC0886]

Description

Rabbit monoclonal [ARC0886] antibody to SKP2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SKP2 (Q13309).

Sequence

MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0886

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDK2/Cyclin A associated protein p45, Cyclin A/CDK2 associated protein p45, Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, F-box protein Skp2, F-box/LRR-repeat protein 1, FBL 1, FBL1, FBXL 1, FBXL1, FLB 1, FLB1, MGC1366, p45, p45skp2, S phase kinase associated protein 2, S phase kinase associated protein 2 (p45), S-phase kinase-associated protein 2, S-phase kinase-associated protein 2 E3 ubiquitin protein ligase, SKP 2, SKP2_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-SKP2 Antibody [ARC0886] (A307422)

Western blot analysis of extracts of HeLa cells, using Anti-SKP2 Antibody [ARC0886] (A307422) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Enlarge Image

Figure 2: Western Blot - Anti-SKP2 Antibody [ARC0886] (A307422)

Western blot analysis of extracts of various cell lines, using Anti-SKP2 Antibody [ARC0886] (A307422) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-SKP2 Antibody [ARC0886] (A307422)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SKP2 Antibody [ARC0886] (A307422) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Publishing research using Anti-SKP2 Antibody [ARC0886] (A307422)? Please let us know so that we can list the citation on this page.