Anti-Smad1 Antibody [ARC52105] (A309165)

Name: Anti-Smad1 Antibody [ARC52105]
Brand: Antibodies.com
SKU: A309165

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This product is discontinued

Anti-Smad1 Antibody [ARC52105] (A309165) has been discontinued and is no longer available.

View all Anti-Smad1 Antibodies.

Specifications

Name

Anti-Smad1 Antibody [ARC52105]

Description

Rabbit monoclonal [ARC52105] antibody to Smad1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_005891.1).

Sequence

WKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52105

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BSP-1, BSP1, HsMAD1, JV4-1, JV41, MAD homolog 1, MAD mothers against decapentaplegic homolog 1, Mad related protein 1, Mad-related protein 1, MADH1, MADR1, Mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMA- AND MAD-RELATED PROTEIN 1, SMAD 1, SMAD family member 1, SMAD mothers against DPP homolog 1, SMAD1_HUMAN, TGF beta signaling protein 1, Transforming growth factor-beta-signaling protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Smad1 Antibody [ARC52105] (A309165)

Western blot analysis of extracts from wild type (WT) and Smad1 knockout (KO) HeLa cells, using Anti-Smad1 Antibody [ARC52105] (A309165) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Smad1 Antibody [ARC52105] (A309165)

Western blot analysis of extracts of HUVEC cells, using Anti-Smad1 Antibody [ARC52105] (A309165) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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