Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Name: Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170]
Brand: Antibodies.com
SKU: A308811
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0170] antibody to Smad3 (phospho Ser423 + Ser425) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170]

Description

Rabbit monoclonal [ARC0170] antibody to Smad3 (phospho Ser423 + Ser425).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S423 & S425 of human Smad3 (P84022).

Sequence

SSVS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0170

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Western blot analysis of extracts of C6 cells, using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at 1:1,000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Smad3 (phospho Ser423 + Ser425) Antibody [ARC0170] (A308811) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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