Anti-Smad4 Antibody (A14886)

Name: Anti-Smad4 Antibody
Brand: Antibodies.com
SKU: A14886
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Smad4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-Smad4 Antibody

Description

Rabbit polyclonal antibody to Smad4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).

Sequence

PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

63 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

(Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, DPC 4, DPC4, hSMAD4, JIP, MAD homolog 4, MAD mothers against decapentaplegic Drosophila homolog 4, MAD mothers against decapentaplegic homolog 4, MADH 4, MADH4, Med, Medea, Mothers against decapentaplegic homolog 4, Mothers against decapentaplegic, Drosophila, homolog of, 4, Mothers against DPP homolog 4, MYHRS, OTTHUMP00000163548, SMA- and MAD-related protein 4, SMAD 4, SMAD family member 4, SMAD mothers against DPP homolog 4, SMAD4_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Immunohistochemistry - Anti-Smad4 Antibody (A14886)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Smad4 Antibody (A14886) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 2: Immunohistochemistry - Anti-Smad4 Antibody (A14886)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Smad4 Antibody (A14886) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Smad4 Antibody (A14886)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Smad4 Antibody (A14886) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Smad4 Antibody (A14886)

Immunofluorescence analysis of C6 cells using Anti-Smad4 Antibody (A14886) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).