Anti-Smek1 Antibody (A16213)

Name: Anti-Smek1 Antibody
Brand: Antibodies.com
SKU: A16213
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Smek1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Smek1 Antibody

Description

Rabbit polyclonal antibody to Smek1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1).

Sequence

VQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLFL1, KIAA2010, MEK1 SUPPRESSOR 1, MSTP033, P4R3A_HUMAN, PP4R3, PP4R3 ALPHA, PP4R3A, PPP4R3A, Protein phosphatase 4 regulatory subunit 3A, PROTEIN PHOSPHATASE 4, REGULATORY SUBUNIT 3, ALPHA, Serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1, SMEK homolog 1, suppressor of mek1 (Dictyostelium), Smk 1, SMK1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Smek1 Antibody (A16213)

Western blot analysis of extracts of various cell lines, using Anti-Smek1 Antibody (A16213) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Smek1 Antibody (A16213)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Smek1 Antibody (A16213) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Smek1 Antibody (A16213)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Smek1 Antibody (A16213) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smek1 Antibody (A16213)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Smek1 Antibody (A16213) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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