Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-SOD2 / MnSOD Antibody (A305174), at 1:120 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion matrix. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-SOD2 / MnSOD Antibody (A305174) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Mitochondrion matrix.
Figure 3: Western Blot - Anti-SOD2 / MnSOD Antibody (A305174)
Western blot analysis of rat tissue lysates showing detection of SOD2 protein using Anti-SOD2 / MnSOD Antibody (A305174) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-SOD2 / MnSOD Antibody (A305174), at 1:120 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion matrix. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.
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