Anti-SOX10 Antibody [ARC1768] (A309023)

Name: Anti-SOX10 Antibody [ARC1768]
Brand: Antibodies.com
SKU: A309023

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This product is discontinued

Anti-SOX10 Antibody [ARC1768] (A309023) has been discontinued and is no longer available.

View all Anti-SOX10 Antibodies.

Specifications

Name

Anti-SOX10 Antibody [ARC1768]

Description

Rabbit monoclonal [ARC1768] antibody to SOX10.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human SOX10 (P56693).

Sequence

VDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1768

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 - 70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DOM, Dominant megacolon mouse human homolog of, MGC15649, PCWH, SOX 10, SOX10_HUMAN, SRY (sex determining region Y) box 10, SRY box 10, SRY box containing gene 10, SRY related HMG box gene 10, Transcription factor SOX 10, Transcription factor SOX-10, WS2E, WS4, WS4C

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SOX10 Antibody [ARC1768] (A309023)

Western blot analysis of extracts of various cell lines, using Anti-SOX10 Antibody [ARC1768] (A309023) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-SOX10 Antibody [ARC1768] (A309023)

Immunofluorescence analysis of C6 cells using Anti-SOX10 Antibody [ARC1768] (A309023) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Western Blot - Anti-SOX10 Antibody [ARC1768] (A309023)

Immunoprecipitation analysis of 300µg extracts of C6 cells using 3µg of Anti-SOX10 Antibody [ARC1768] (A309023). This Western blot was performed on the immunoprecipitate using Anti-SOX10 Antibody [ARC1768] (A309023) at a dilution of 1:1000.

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