Anti-SOX9 Antibody [ARC0190] (A306215)

Name: Anti-SOX9 Antibody [ARC0190]
Brand: Antibodies.com
SKU: A306215
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0190] antibody to SOX9 for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Neural Stem Cell Marker

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Specifications

Name

Anti-SOX9 Antibody [ARC0190]

Description

Rabbit monoclonal [ARC0190] antibody to SOX9.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 169-300 of human SOX9 (P48436).

Sequence

HPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0190

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

56 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

campomelic dysplasia autosomal sex reversal, CMD 1, CMD1, CMPD 1, CMPD1, SOX 9, SOX9_HUMAN, SRA 1, SRA1, SRXX2, SRXY10, SRY (sex determining region Y) box 9, SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal, SRY (sex determining region Y)-box 9, SRY (sex-determining region Y)-box 9 protein, SRY related HMG box gene 9, Transcription factor SOX 9, Transcription factor SOX-9, transcription factor SOX9

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-SOX9 Antibody [ARC0190] (A306215)

Western blot analysis of extracts of various cell lines, using Anti-SOX9 Antibody [ARC0190] (A306215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-SOX9 Antibody [ARC0190] (A306215)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-SOX9 Antibody [ARC0190] (A306215) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SOX9 Antibody [ARC0190] (A306215)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SOX9 Antibody [ARC0190] (A306215) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SOX9 Antibody [ARC0190] (A306215)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-SOX9 Antibody [ARC0190] (A306215) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.