Anti-SP1 Antibody [ARC0128] (A308986)

Name: Anti-SP1 Antibody [ARC0128]
Brand: Antibodies.com
SKU: A308986
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0128] antibody to SP1 for WB, IHC, ICC/IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SP1 Antibody [ARC0128]

Description

Rabbit monoclonal [ARC0128] antibody to SP1.

Applications

WB, IHC, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SP1 (P08047).

Sequence

TTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0128

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

SP 1, Sp1 transcription factor, SP1_HUMAN, Specificity protein 1, Transcription factor Sp1, TSFP 1, TSFP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SP1 Antibody [ARC0128] (A308986)

Western blot analysis of extracts of various cell lines, using Anti-SP1 Antibody [ARC0128] (A308986) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-SP1 Antibody [ARC0128] (A308986)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-SP1 Antibody [ARC0128] (A308986) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SP1 Antibody [ARC0128] (A308986)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SP1 Antibody [ARC0128] (A308986) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.