Anti-SPARC Antibody (A89696)

Name: Anti-SPARC Antibody
Brand: Antibodies.com
SKU: A89696
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SPARC for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-SPARC Antibody

Description

Rabbit polyclonal antibody to SPARC.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).

Sequence

APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AA517111, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, BM40, Cysteine rich protein, hm:zeh0062, MGC128090, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine, Secreted protein acidic cysteine rich, Secreted protein acidic cysteine rich (osteonectin), SPRC, SPRC_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SPARC Antibody (A89696)

Western blot analysis of extracts of A375 cells, using Anti-SPARC Antibody (A89696) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-SPARC Antibody (A89696)

Immunofluorescence analysis of C6 cells using Anti-SPARC Antibody (A89696) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-SPARC Antibody (A89696)

Immunofluorescence analysis of L-929 cells using Anti-SPARC Antibody (A89696) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-SPARC Antibody (A89696)

Immunofluorescence analysis of U-2 OS cells using Anti-SPARC Antibody (A89696) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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