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Anti-Spt6 Antibody (A88931)

Western Blot - Anti-Spt6 Antibody (A88931) - Antibodies.com Immunofluorescence - Anti-Spt6 Antibody (A88931) - Antibodies.com Western Blot - Anti-Spt6 Antibody (A88931) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Spt6 for WB, ICC/IF and IP.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Spt6 Antibody
Description
Rabbit polyclonal antibody to Spt6.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1500-1726 of human SUPT6H (NP_003161.2).
Sequence
IFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
260 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
emb 5, hSPT6, KIAA0162, MGC87943, SPT6H, SPT6H_HUMAN, Suppressor of Ty (S.cerevisiae) 6 homolog, Suppressor of Ty 6 homolog (S. cerevisiae), SUPT6H, Tat cotransactivator 2 protein, Tat CT2 protein, Tat-cotransactivator 2 protein, Tat-CT2 protein, Transcription elongation factor SPT6
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Spt6 Antibody (A88931) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Spt6 Antibody (A88931)
Western blot analysis of extracts of various cell lines, using Anti-Spt6 Antibody (A88931) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-Spt6 Antibody (A88931) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Spt6 Antibody (A88931)
Immunofluorescence analysis of U-2 OS cells using Anti-Spt6 Antibody (A88931) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Spt6 Antibody (A88931) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Spt6 Antibody (A88931)
Immunoprecipitation analysis of 600µg extracts of mouse heart using 3µg of Anti-Spt6 Antibody (A88931). This Western blot was performed on the immunoprecipitate using Anti-Spt6 Antibody (A88931) at a dilution of 1:500.
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