Anti-SQSTM1 / p62 Antibody (A305009)

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3 (mouse fibroblast cell line), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-SQSTM1 / p62 Antibody (A305009), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Late Endosome, Lysosome, Cytoplasmic Vesicle, Autophagosome, Nucleus, Endoplasmic Reticulum. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SQSTM1 Antibody (D) Composite.

Western blot analysis of human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Anti-SQSTM1 / p62 Antibody (A305009) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~65 kDa in all lysates.