Anti-Src Antibody (A81182)

Name: Anti-Src Antibody
Brand: Antibodies.com
SKU: A81182
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Src for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Src Antibody

Description

Rabbit polyclonal antibody to Src.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human Src (NP_005408.1).

Sequence

MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ASV, Avian sarcoma virus, c SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, cSrc, EC 2.7.10.2, Neuronal CSRC tyrosine specific protein kinase, Neuronal SRC, Oncogene SRC, OTTHUMP00000174476, OTTHUMP00000174477, p60 Src, p60-Src, p60Src, pp60c src, pp60c-src, pp60csrc, Proto oncogene tyrosine protein kinase Src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, Protooncogene SRC Rous sarcoma, SRC Oncogene, SRC proto oncogene non receptor tyrosine kinase, SRC1, SRC_HUMAN, Tyrosine kinase pp60c src, Tyrosine protein kinase SRC 1, Tyrosine protein kinase SRC1, v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog, V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian), v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Src Antibody (A81182)

Western blot analysis of extracts of various cell lines, using Anti-Src Antibody (A81182) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Src Antibody (A81182)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Src Antibody (A81182) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Src Antibody (A81182)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Src Antibody (A81182) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Src Antibody (A81182)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Src Antibody (A81182) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.