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Anti-SRD5A2 Antibody [ARC2287] (A307681)

Western Blot - Anti-SRD5A2 Antibody [ARC2287] (A307681) - Antibodies.com Immunofluorescence - Anti-SRD5A2 Antibody [ARC2287] (A307681) - Antibodies.com Immunofluorescence - Anti-SRD5A2 Antibody [ARC2287] (A307681) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2287] antibody to SRD5A2 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-SRD5A2 Antibody [ARC2287]
Description
Rabbit monoclonal [ARC2287] antibody to SRD5A2.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213).
Sequence
MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2287
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
3 oxo 5 alpha steroid 4 dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha SR2, 5 alpha-SR2, 5ART2, e II 5 alpha reductase, EC, MGC138457, Micropenis, included, S5A2_HUMAN, S5AR 2, SR type 2, Steroid 5 alpha reductase 2, Steroid 5-alpha-reductase 2, Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2), Type II 5-alpha reductase
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SRD5A2 Antibody [ARC2287] (A307681) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SRD5A2 Antibody [ARC2287] (A307681)
Western blot analysis of extracts of various cell lines, using Anti-SRD5A2 Antibody [ARC2287] (A307681) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-SRD5A2 Antibody [ARC2287] (A307681) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-SRD5A2 Antibody [ARC2287] (A307681)
Immunofluorescence analysis of rat testis using Anti-SRD5A2 Antibody [ARC2287] (A307681) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-SRD5A2 Antibody [ARC2287] (A307681) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-SRD5A2 Antibody [ARC2287] (A307681)
Immunofluorescence analysis of mouse testis using Anti-SRD5A2 Antibody [ARC2287] (A307681) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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